Acetylcholinesterase Inhibitory Activity of Standardized Cannabinoids-rich Fractions

Background: Since cannabis has been legally allowed for medicinal purposes in many countries, it become the most interesting issue, particularly neurologic disorders, such as Alzheimer’s disease (AD). Inhibition of acetylcholinesterase (AChE) is one mechanisms treatment AD. Objectives: The present study aimed to establish a method preparation cannabinoid-rich extracts and determine their AChE inhibitory activity. Methods: were prepared through green extraction process using microwave-assisted (MAE) followed by hydrophobic column separation. contents cannabidiol (CBD) Δ-9-tetrahydrocannabinol (THC) determined high-performance liquid chromatography (HPLC). In vitro activity was via photometric from Electrophorus electricus. Results: Three cannabinoids-rich fractions obtained with different concentrations CBD THC, namely Fractions I (CBD 8.1% w/w; THC 52.2% w/w), II 9.2% 8.0% III 1.3% w/w, 33.5% w/w). These exhibited activity, IC50 values 52.3, 59.8, 71.2 µg/mL, respectively. Conclusion: This finding suggests that CBD, but not might be an active compound contributing effect.